A sensitive solid–phase micro–spot radio–tracer assay for the simultaneous estimation of thyroid stimulating hormone and thyroglobulin.

Bharti Jain; Savita Kulkarni; C.Gholve; J.Kumarasamy; M.G.R.Rajan

Volume : V, Issue : IX, September - 2015

A sensitive solid–phase micro–spot radio–tracer assay for the simultaneous estimation of thyroid stimulating hormone and thyroglobulin.

Bharti Jain, Savita Kulkarni, C. Gholve, J. Kumarasamy, M. G. R. Rajan

Abstract :

Hormones and tumour–markers in blood are assayed using immunoassays since this technique offers high sensitivity and specificity. Clinical diagnosis usually requires the blood concentration of several related analytes. Since immunoassays for each analyte is performed individually, estimating multiple analytes take time and are costly. This need can be fulfilled by use of multi–analyte immunoassay (MAIA). Using this concept, we have developed immunoradiometric assays for the simultaneous estimation of thyroid stimulating hormone (TSH) and thyroglobulin (Tg) in serum samples. Highly micro–porous polycarbonate (PC) track–etched memane (TEM) was used as a solid support for immobilization of the anti–TSH and anti–Tg antibodies. 125I labeled antibodies were used for detection. The assays were validated and compared to established methods. Assays are found to be sensitive, with wide range and with acceptable precision. Significant correlation was seen r=0.98, p < 0.001 for TSH and r= 0.91, p < 0.001 for Tg when compared to established methods