Volume : III, Issue : VII, July - 2013

A kinetic study of the rate reaction of gamma–glutamyltransferase in hepatitis patients

Namama Soran H. Al Talabani

Abstract :

Serum GGT activity has been investigated in hepatitis, activities were found elevated in this case studied at basic pH and 37 C0.The study was concentrated to comprehensive determination of the rate reaction kinetics of the enzyme reaction in both normal and hepatitis sera. The pseudo first –order plot reflects both values of the first–order association constant K1 and the half life time (t1/2) of the enzymatic reaction. The activation energy of the reaction (EScomplex formation) with Hill coefficient (n) were both estimated using Arrhenius and Hill plots respectively. The activation energy E* for GGT reaction decreased in hepatitis patients compå with normal group ,and the (ΔH* and ΔG*) values for GGT enzyme in normal and hepatitis were positive, while ΔS* value for GGT enzyme in normal and hepatitis were negative. These data showed that in hepatitis patients there was a change in the mechanism of the ES– complex formation in the GGT enzyme reactions.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Namama Soran H. Al-Talabani A kinetic study of the rate reaction of gamma–glutamyltransferase in hepatitis patients Indian Journal of Applied Research, Vol.III, Issue.VII July 2013

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