SIMULTANEOUS DETERMINATION OF METFORMIN AND REPAGLINIDE IN BULK AND TABLETS BY RP-HPLC METHOD

Desabattina Sai Jahnavi; Desabattina Venkata Narayana Rao; Yeluri Ramachandra Reddy

Volume : V, Issue : X, October - 2016

SIMULTANEOUS DETERMINATION OF METFORMIN AND REPAGLINIDE IN BULK AND TABLETS BY RP-HPLC METHOD

Desabattina Sai Jahnavi, Desabattina Venkata Narayana Rao, Yeluri Ramachandra Reddy

Abstract :

The present communication describes a rapid, precise and accurate RP-HPLC method for the simultaneous quanti�cation of metformin and repaglinide in bulk and tablet dosage forms. Chromatographic separation of the selected drugs was carried out on Phenomenex C18 analytical column (250 mm x 4.6 mm, 5 μ particle size). An isocratic elution system was developed using 0.1M ammonium acetate: acetonitrile [50:50 v/v]. The pH of the mobile was adjusted to 4.2 with orthophosphoric acid. The elution of the drugs was achieved with a �ow rate of 1.0 ml/min. Detection was by UV absorbance at a wavelength of 254 nm. The detector response was linear in the concentration of 500-1500 �g/ml and 2-6 �g/ml for metformin and repaglinide, respectively. The method was validated following ICH guidelines. All the parameters of validation were found in the acceptance range.