Prevalence of microorganisms in Microbial  Keratitis and their  antibiotic and antifungal sensitivity pattern.

SARATHA D

Volume : VI, Issue : V, May - 2017

Prevalence of microorganisms in Microbial Keratitis and their antibiotic and antifungal sensitivity pattern.

Saratha D

Abstract :

Aim:Infectious keratitis is a vision threatening condition.If it is not properly diagnosed and treated with appropriate anti micribials, it will lead to loss of vision.The purpose of the study is to determine the prevalence of microorganisms & antibiotic and anti fungal sensitivity pattern of microorganisms involved in keratitis .Materials &Methods:From 120 patients with corneal ulcer a total of 75 organisms [bacteria & fungi] were islated & subjected for antibacterial and anti fungal sensitivity testing.Antibiotic sensitivity testing was carried out by disc diffution method by using antibiotics such as Gatifloxacin, Toamycin,Ceftazidime,Vancomycin &Cotrimoxazole.Antifungal sensitivity pattern was carried out by disc diffusion method by using Amphotericin B,Itraconazole &Flucanazole. Results:All the bacterial isolates were 100% sensitive to Vancomycin .88% of S.pneumoniae spp,85% of Pseudomonas ,75% of Nocardia and 50% S.Viridans were sensitive to Gatifloxacin ,88% of S.Pneumoniae,and 71% of Pseudomonas ,50% of Nocardia and 50% of S.Viridans were sensitive to Toamycin ,55.55% Of S.Pneumoniae ,42.8% of Pseudomonas 50% of Nocardia and 50% of S.Viridans were sensitive to Ceftazidime.Anti fungal sensitivity pattern of fungal isolates done by disc diffusion method showed that 71% Fusarium, 62% of A.Flavus,50% of A.fumigatus ,66.6% of A.niger,50% of Bipolaris were sensitive to Amphotericin B,82% of Fusarium ,75% of A.flavus,75% of A.fumigatus and 66.6% of A.niger and 50% of Bipolaris were sensitive to Itraconazole.All the organisms were resistant to flucanazole by disc diffustion method.Conclusion:90% of the bacterial isolates were sensitive to toamycin followed by Gatifloxacin [88%] and Ceftazidime[55%]. All were sensitive to Vancomycin. Among the fungal isolates ,85% of them were sensitive to Amphotericin B and 90% of them were sensitive to Itraconazole.All the fungal isolates were resistant to Flucanazole by disc diffusion method.Knowledge of antimicrobial resistance is essential as it has prognostic value in the final clinical and visual outcome.