Optimization of extraction method and profiling of plant phenolic compounds of Merremia umbellata (Hallier. f.)through RP-HPLC

Mrs.K.Kaveri; Dr. Jeyabalan Govidasamy

Volume : VIII, Issue : VII, July - 2019

Optimization of extraction method and profiling of plant phenolic compounds of Merremia umbellata (Hallier. f.)through RP-HPLC

Mrs. K. Kaveri, Dr. Jeyabalan Govidasamy

Abstract :

The Merremia umbellata (Hallier. f.) phenolics extraction efficiency of CAE, MAE and UAE methods were assessed by comp� their RP-HPLC chromatogram the highest recovery of phenolic compounds in terms of number and yield was obtained in CAE when compared with MAE and UAE. The CAE is traditionally successful extraction method because in this method, maceration or percolation mechanism accounts for better extraction efficiency Phenolic compounds were monitored in the range between 280, 300 to 320 nm . Among these monitoring wavelengths, at 280 nm, most of the abundant phenolic compounds were detected. It is because most of the phenolic compounds absorbance maximum is near to 280 nm.