Molecular phylogeny of Sesuvium portulacastrum using 18S nuclear ribosomal gene sequence

Snehal  B.Gagare; Pratima S. Jadhav

Volume : VI, Issue : V, May - 2017

Molecular phylogeny of Sesuvium portulacastrum using 18S nuclear ribosomal gene sequence

Snehal B. Gagare, Pratima S. Jadhav

Abstract :

Mangroves are halophyte plants which exist in conditions of high salinity, extreme tides, strong winds and high temperature, muddy and anaerobic soils. Sesuvium portulacastrum is a mangrove species in the genus Sesuvium of the family Aizoaceae. Nuclear 18S rDNA gene is one of the most important molecular markers, used in diverse applications such as molecular phylogenetic analyses and biodiversity screening. In the present study Nuclear 18S rDNA gene of Sesuvium portulacastrum was amplified and sequenced. The sequence was submitted to NCBI genebank with accession no KC 146551.The length and GC percent of the Nuclear 18S rDNA gene was also calculated .It was found to be 1638 base pairs (bp) in length where as GC% found 51.2. There are 838 GC out of 1638 bases. From Phylogenetic tree it is inferred that Sesuvium portulacastrum is closely related to Pereskia aculeate and Basella alba. Along with these two species Sesuvium portulacastrum forms monophyletic group with other closely related species such as Opuntia sp, Claytonia perforliata, Portulaca grandiflora.