Molecular aspect of multi-drug resistant extended spectrum beta-lactamases (ESBL) secreting Enterobacteriaceae strains isolated at both bacteriology laboratory

Elhadji Bambo DIAKHABY; Halimatou DIOP-NDIAYE; Ana Julienne Selbe NDIAYE; Edgard Adam Macondo; Makhtar CAMARA; Tidiane SIBY; Cheikh Saad Bouh BOYE; Coumba TOURE-KANE

Volume : IX, Issue : V, May - 2020

Molecular aspect of multi-drug resistant extended spectrum beta-lactamases (ESBL) secreting Enterobacteriaceae strains isolated at both bacteriology laboratory

Elhadji Bambo Diakhaby, Halimatou Diop Ndiaye, Ana Julienne Selbe Ndiaye, Edgard Adam Macondo, Makhtar Camara, Tidiane Siby, Cheikh Saad Bouh Boye, Coumba Toure Kane

Abstract :

The objective of this work was to study at a molecular level the resistance genes of the ESBL-producing enterobacterial strains isolated at the Laboratory of Bacteriology and Virology of the CHNU Aristide Le Dantec and at the private laboratory Bio-24. The CTXM genes of group 1 (CTXM1) and the CTXM of group 9 (CTXM9) encoding for ESBL, OXA1 and CMY1 encoding for penicillinase and cephamycinase respectively were searched by PCR followed by nucleotide sequencing. The resulting sequences were submitted to the NCBI-Blast site for gene typing. The CTXM genes in Group1, CTXM in Group 9, OXA1 and CMY1 were found in 96%, 18%, 85% and 56% of the strains, respectively. Nucleotide sequencing of 15 CTXM1 allowed the CTXM15 to be recovered. These resistance genes can be associated with those of quinolones and/or carbapenems and thus lead to a therapeutic impasse, hence the need to strengthen the fight against antibiotic resistance.