Microbiological scrutiny of operation theatre in multi- speciality Hospitals in Gwalior, Madhya Pradesh

Shilpi Gupta; Naveen Gupta

Volume : VIII, Issue : V, May - 2019

Microbiological scrutiny of operation theatre in multi- speciality Hospitals in Gwalior, Madhya Pradesh

Shilpi Gupta, Naveen Gupta

Abstract :

Background: Good hospital hygiene is an essential part of infection control programme. Bacterial counts in operation theatres are predisposed by number of individual present, ventilation and air flow methods. Objective: To determine prevalence rate of microorganisms in Operation Theatre and to find out the incidence of contamination from various locations in operation theatre. Methods: The study was conducted in the department of microbiology and Pathology Laboratory of Multi- Speciality Hospitals at Gwalior. Air sampling was done, and samples were taken by a sterile swab saturated in nutrient oth from all operation theatres. The samples were administered according to standard operative procedures. Analysis was done the Epi-info 7 software. Results: Least bacterial colony forming unit (CFU) was revealed by ophthalmology OT 21CFU/mm3 and highest was shown by emergency OT 196 CFU/mm3. Isolated organism was separated into normal flora (CONS, micrococci), contaminant (bacillus species) and pathogenic organism e.g. Staphylococcus aureus, Acinetobacter. Conclusions: Continuous Scrutiny and laboratory capacity will surely improve infection prevention and control. Routine sampling is strongly recommended for increasing awareness to identify and control all possible bases and types of infections.