Microbiological profile and antibiotic susceptibility pattern of pus isolates from a tertiary care hospital

Minu Bali; Subhash Chand Jaryal; Anuradha Sood; Nanish Sharma

Volume : IX, Issue : VI, June - 2020

Microbiological profile and antibiotic susceptibility pattern of pus isolates from a tertiary care hospital

Minu Bali, Subhash Chand Jaryal, Anuradha Sood, Nanish Sharma

Abstract :

Background and aim: Profile of microbiological agents and their susceptibility to different antibiotics have to be understood before st� proper treatment. The study was aimed to obtain microbiological profile of pus isolates, and to ascertain antibiotic susceptibility pattern of pus isolates. Methods: A retrospective study was conducted in a tertiary care hospital, Dr. RPGMC Tanda. A total of 570 pus culture samples collected by sterile swab and sterile syringe aspiration from clinically suspected cases of pyogenic infections, from different wards, were processed by standard microbiological methods. Antibiotic susceptibility testing was done by Kirby-Bauer disc diffusion test as per CLSI guidelines and were classified as sensitive or resistant. Results: Out of 570 samples received in the department of Microbiology, Dr. RPGMC, Tanda for antibiotic culture and sensitivity, 288 (50.5%) samples yielded positive culture whereas 282 (49.5%) samples yielded no growth. Among 570 samples, 554 (97.1%) were adults whereas 16 (3.9%) were children. Out of 570 samples, 393 were males while 177 were females. Samples were mainly from orthopedics ward (52.6%) and surgery ward (21%) followed by medicine, obstetrics and dermatology wards. Growth was mono-microbial in 204 (70.8%) isolates, bi-microbial in 72 (25%) while poly- microbial in 12 (4.2%) pus isolates. Staphylococcus aureus was the most common gram-positive organism. Prevalence of MRSA was 66.84% among Staphylococcus. Among gram negative organisms, E. coli was most common followed by Pseudomonas species. Candida was the only fungal growth seen in 10 (2.6%) samples. Antibiotic susceptibility pattern of Staphylococcus showed 100% susceptibility to vancomycin. Among the AST profile of gram-negative organisms, E. coli showed 76.1% sensitivity to aminoglycosides while Pseudomonas showed 88.5% sensitivity to piperacillin. Conclusion: A continuous inspection should be carried out to monitor the antimicrobial susceptibility of bacterial isolates from pus samples to choose appropriate antibiotics for prophylaxis and treatment of infections.