M. tuberculosis in Bone Marrow as commensal � A hidden source for primary osteoarticular Tuberculosis !!

Vidyut Prakash; Deepak Kumar; Saurabh Singh; Gopal Nath

Volume : VI, Issue : VII, July - 2017

M. tuberculosis in Bone Marrow as commensal � A hidden source for primary osteoarticular Tuberculosis !!

Vidyut Prakash, Deepak Kumar, Saurabh Singh, Gopal Nath

Abstract :

BACKGROUND:

The bone marrow of healthy individuals is conventionally considered sterile like other body fluids, but recently the scientists found genetic material from the bacteria inside their stem cells. The findings raise the possibility that other infectious agents may also reside in the bone marrow.

OBJECTIVES:

The study was planned to look for the presence of all type of bacteria by amplifying 16S rDNA sequences by using eubacterial universal primers.

MATERIAL AND METHODS:

A total of 18 bone marrow samples of apparently healthy individuals were collected from patients admitted having closed bone fractures. The DNA was isolated and was subjected to nested PCR using Universal eubacterial 16S rDNA primers. The samples positive by universal PCR was further checked for the presence of S. Typhi, S. ParaTyphi, A and or M. tuberculosis if any using a second nested PCR reaction.

RESULTS:

A total of 16 (89%) samples could yield the desired amplicon through universal PCR. The secondary PCR of 16 samples, the desired amplicons were detected 3 (18%) for M. Tuberculosis, 4 (22%) for S.Typhi, and 1 (6%) for ParaTyphi A.

CONCLUSIONS:

Even in asymptomatic cases other infectious agents like M. tuberculosis, Salmonella spp. and many other microorganisms may be present as commensal and may cause active clinical infection on certain conditions.