Volume : V, Issue : I, January - 2016

Isozyme as Biomarker for Identification of Rice varieties

Rimpi Bora, Sharmilla Dutta Deka

Abstract :

Rice varieties of Assam, which are in seed production chain, lack in compilation of key diagnostic characters. Systematic studies to develop diagnostic markers of these varieties are essential which are morphologically very similar, to carryout scientific seed production, certification and also for plant variety protection. Eleven rice varieties of Assam, which are in high demand in seed chain were characterized based on SDS-PAGE of total soluble protein and two isozyme systems viz., esterase and aspartate amino transferase to use as biomarker for rapid identification of the varieties.. The electrophoretic banding profile of total protein exhibited no specific bands for identification of the varieties. However, variations were observed among the varieties with respect to Esterase banding pattern. Varieties could be grouped into five clusters in the dendrogram based on isozymes. Among the two isozyme systems, esterase isozyme was found to be more suitable for identification of the rice varieties.

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Article: Download PDF    DOI : https://www.doi.org/10.36106/gjra  

Cite This Article:

Rimpi Bora, Sharmilla Dutta Deka Isozyme as Biomarker for Identification of Rice varieties Global Journal For Research Analysis, Vol: 5, Issue: 1 January 2016

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