ISOLATION, SCREENING AND CHARACTERIZATION OF ORGANIC ACIDS PRODUCING BACTERIA FROM NOVEL SOURCE

Mili Sandip Shah

Volume : VIII, Issue : I, January - 2019

ISOLATION, SCREENING AND CHARACTERIZATION OF ORGANIC ACIDS PRODUCING BACTERIA FROM NOVEL SOURCE

Mili Sandip Shah

Abstract :

Microbial production of organic acids is a promising approach for obtaining building-block chemicals from renewable carbon sources. Although some acids have been produced for some time and in-depth knowledge of these microbial production processes has been gained, further microbial production processes seem to be feasible, but large-scale production has not yet been possible. The study aim involves isolation, screening, purification and characterization of organic acids from bacterial origin. For increased yield, various and varying sources of carbon, nitrogen including physical parameters influencing the growth and yield viz. temperature, pH were studied for optimizations of medium ingredients. Maximum production in terms of zone of production was obtained when medium was incorporated with Sucrose (3.0g%) and yeast extract (0.7g%) with pH values 6.3 at 42�C. Organic acids from crude extracts were detected using Rf values by performing thin layer chromatography along with standards alongside the sample in a mobile phase, referred to as the eluent, consisting of EtOH:NH4OH:H2O mixture (75.5:12.5:12, v/v/v) resulting in the progressive separation of the organic acids. Organic acids detection reveals spots corresponding to salicylic acid (Rf 0.78), tartaric (Rf 0.17) and succinic (Rf 0.44). Organic acid detection is to spray the plate with 0.4% (w/v) omocresol green in ethanol, in which were added few drops of 0.1 N NaOH, which result in colored spots. Succinic, tartaric and salicylic acid is the most important in tonnage having extensive applications in food and pharmaceutical industries