Volume : VI, Issue : VIII, August - 2017

INVITRO ASSAY FOR THE SPECIFIC ACTIVITY OF HUMAN SALIVARY AMYLASE ON STARCH

Dr. Padmanabha. B

Abstract :

 Objective: is to estimate the specific activity of starved and unstarved human salivary amylase on starch invitro.

Materials and methods: Saliva collected from starved and unstarved humans subjected to Protein estimation and hydrolytic activity on starch.

Results and discussion: Experimental evidences suggested that, 1ml of starved human saliva contains more (74µg) protein than unstarved human saliva (60 µg). Starved human saliva hydrolysed starch in to maltose more (400mg) than unstarved human saliva (250mg). According to this invitro study, the Specific activity of 1 ml of starved salivary amylase is (1.0 micro mole/mg /min) higher than the unstarved human ptyalin (0.771µ mole/mg^-1/min^-1) on starch.

Conclusion: The saliva of starved humans has higher ptyalin and specific activity than unstarved humans.

Keywords :

Article: Download PDF    DOI : https://www.doi.org/10.36106/gjra  

Cite This Article:

Dr. Padmanabha. B, INVITRO ASSAY FOR THE SPECIFIC ACTIVITY OF HUMAN SALIVARY AMYLASE ON STARCH, GLOBAL JOURNAL FOR RESEARCH ANALYSIS : VOLUME-6, ISSUE-8, AUGUST-2017

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