Infuence of Polymethylene chain length and choline head cyclization on dicholine esters hydrolysis by human butyrylcholinesterase: an NMR approach.

Debouzy Jean-Claude; Nugue Guillaume; Selek Laurent; Crouzier Davida

Volume : IV, Issue : X, October - 2015

Infuence of Polymethylene chain length and choline head cyclization on dicholine esters hydrolysis by human butyrylcholinesterase: an NMR approach.

Debouzy Jean Claude, Nugue Guillaume, Selek Laurent, Crouzier Davida

Abstract :

The butyrilcholinesterase-induced enzymatic hydrolysis of a dicholine ester derivative (A4), be� a methyl piperidine head group instead of a choline was studied by 1H-NMR spectroscopy. The various enzyme/substrate molar ratios and concentrations allowed to determine an estimation of K�m of 0,1mM. A4 hydrolysis time course was compared to dicholine derivatives with either a similar acyl chain (4 carbons for DCH4), or similar overall length (DCH8, with a 8 carbon chain). Although a similar initial kinetic slope was found for the three molecules, later evolution was found very close for DCH8 and A4, while slower and more incomplete for A4. These results are discussed in terms of overall physicochemical properties and global hindrance