Volume : IV, Issue : IX, September - 2015

INFLUENCE OF DIETARY ASCORBIC ACID INTAKE IN WOMEN BIDI ROLLERS BY COMET ASSAY

P. Minny Jael, K. Rudrama Devi

Abstract :

A study was conducted on a group of employees from small scale bidi rolling industries in three different districts of Telangana state were tested for comet tail lengths that are well established as indicators of early biological effects. To investigate whether occupational exposure to tobacco dust is genotoxic, a total of 182 women bidi workers and 182 control groups of individuals in the age group of 16 to 65 years and 6-30 yrs of tobacco dust exposure were recruited; a questionnaire based survey was conducted. In the present study, the assessment of Primary DNA damage hosted by peripheral blood leukocytes of workers employed in tobacco based bidi rolling industry was performed using the alkaline comet assay, the tail length and long-tailed nuclei thereby being the primary outcome of the measure. A significant increase in the incidence of DNA damage was observed in the experimental subjects when compared to their respective controls. A population monitoring study was conducted in human lymphocytes by analysis of comet tail length to investigate whether occupational exposure to tobacco dust is genotoxic in women bidi rollers exposed to tobacco work environment. There was significant increase in the frequency of chromosomal aberrations in human lymphocytes.of women bidi rollers. In addition, a study on the antimutagenic effect of Vitamin C administered orally to women bidi rollers exposed to tobacco dust was investigated by measuring the frequency of comet tail length had a three month daily intake of Vitamin C. The results clearly showed a significant reduction on comets frequency evaluated after vitamin-C treatment. The results of the present study indicate the The processing of tobacco leaves generates a lot of dust and facilitates the release of numerous tobacco components in to ambient air. The results obtained in this investigation indicate that bidi rollers seem to be facing the occupational hazard of genotoxicity due to inhalation and handling of bidi tobacco dust. AA supplementation plays an important role in inhibiting the DNA damage in women bidi rollers exposed to tobacco dust useful findings to public health as lakhs of women are engaged in bidi rolling

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Article: Download PDF    DOI : https://www.doi.org/10.36106/gjra  

Cite This Article:

P. Minny Jael, K. Rudrama Devi Influence of Dietary Ascorbic Acid Intake in Women Bidi Rollers by Comet Assay Global Journal For Research Analysis, Vol: 4, Issue: 9 September 2015

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