IN VITRO ANTI ADIPOGENESIS ASSAY OF KARIVEPPILAI CHOORANAM ON 3T3-L1 CELL LINE

Manoharan A; Chittibabu CV; Manjula M

Volume : VIII, Issue : IX, September - 2019

IN VITRO ANTI ADIPOGENESIS ASSAY OF KARIVEPPILAI CHOORANAM ON 3T3-L1 CELL LINE

Manoharan A, Chittibabu Cv, Manjula M

Abstract :

The adiposeness is a chronic metabolic disorder to affecting people worldwide, with significant morbidity and mortality caused by its micro vascular and macro vascular complications, affecting various important target organs and structures in humans. Recent scientific studies and newer trial drugs have been proved that early detection of hyperlipidaemia will decrease the number of severity in hyperlipidaemic complications. According to ICMR-INDIA B (phase I) study restricted to urban and rural study populations in 4 states in India was hyperlipidemia in 13.9%, low HDL cholesterol in 72.3% and high LDL Cholesterol in 11.8% (Gupta 2010 &Joshi2014) and NCEP-ATP III is observed the prevalence of hyperlipidemia is affected higher in males then in females (Sawant et al). The siddha medicines are well management of reducing weight loss and other complications. Aim: The present study was carried out to determine the anti adipogenic activities of kariveppillai choornam using Mouse Emyo Fioblast 3T3-L1 cell line. Methods and Material: The study is performed to assess the kariveppilai chooranam for its anti-hyperlipedemia by oil red o and triglycerides estimation methods. The test drug kariveppillai choornam is a poly herbal formulation containing eight plants. Results: the kariveppilaichooranam is inhibiting lipid accumulation (anti adipogenesis) by 25.1% and 16.6% over control at 200 �g/ml and 100�g/ml respectively. simultaneously, glycerol released was found to be 119.82�0.90% and 134.53�1.38% over control at 200�g/ml and 100 �g/ml respectively.