IMMUNOHISTOCHEMISTRY ON HELICOBACTER PYLORI: An institutional diagnostic hands-on review

Karthick Prabhu R; Alamelu Jayaraman

Volume : VI, Issue : IX, September - 2017

IMMUNOHISTOCHEMISTRY ON HELICOBACTER PYLORI: An institutional diagnostic hands-on review

Karthick Prabhu R, Alamelu Jayaraman

Abstract :

BACKGROUND: H.Pylori infection is chronic &the prime risk factor for peptic ulcer disease, gastric cancer & primary B-cell lymphomas. It is classified recently by WHO as a CLASS I carcinogen.

OBJECTIVE:1.To review the use of anti H.pylori antibody 2.To compare the efficacy of IHC & special stains in patients with H.pylori associated chronic gastritis.

METHODS: A retrospective study involving 55 cases of H.pylori associated chronic gastritis. Under microscopy, the density of H.pylori,efficacy and sensitivity of the immunohistochemical staining were assessed.

RESULTS: Antibody against H.pylori showed higher sensitivity on comparison with routine H&E and modified Giemsa.

CONCLUSION: Use of H.pylori antibody along with the routine H&E and Modified Giemsa would increase the sensitivity, specificity and decrease the false positive results.