Volume : VIII, Issue : IV, April - 2019

GLYCATED HAEMOGLOBIN AND VARIOUS FRACTIONS ON HPLC

Dr. Mrinal Gupta

Abstract :

Background- Haemoglobin A1c (HbA1c) test has been a major boost in diagnosis & management of diabetes and its estimation has become an important part for the management of diabetes. The term glycated haemoglobin is essentially composed of haemoglobin A1 (HbA1) and HbA1c. Objective of our study was to find out the relationship between sub fractions of glycated haemoglobin and the major fraction,HbA1c and the role of P3 fraction in the final reported value of HbA1c using HPLC. Method- HbA1c,various fractions of HbA1 and P3 were estimated by HPLC method in 430 subjects. Patient data was divided into 3 groups on the basis of HbA1c%. Result- HbA1a%, HbA1b% fraction increases with increase in HbA1c% followed by HbA1a area and HbA1b area. Increase in P3% as well as area was also observed with increase in HbA1c. Conclusion- Sub fractions of glycated haemoglobin and P3 fraction influence the final reported value of HbA1c by HPLC. P3 fraction might indicate the possible presence of variant haemoglobins in the sample.

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Article: Download PDF    DOI : https://www.doi.org/10.36106/gjra  

Cite This Article:

GLYCATED HAEMOGLOBIN AND VARIOUS FRACTIONS ON HPLC, Dr. Mrinal Gupta GLOBAL JOURNAL FOR RESEARCH ANALYSIS : Volume-8 | Issue-4 | April-2019

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