Genistein substantiate and fortifyoxidant potential of a cell by interacting with Bcl-2: an anti-apoptotic protein in rat model.

HumairaFarooqi; Kailash Singh; Ajaz Ahmad Ganai

Volume : IV, Issue : I, January - 2015

Genistein substantiate and fortifyoxidant potential of a cell by interacting with Bcl-2: an anti-apoptotic protein in rat model.

Humairafarooqi, Kailash Singh, Ajaz Ahmad Ganai

Abstract :

Genistein, an isoflavonoid isknown for its antioxidant property. It is known that the presence of Bcl–2 is important for the antioxidant action of genistein. The possible mechanism of action of the genistein is by its interaction with Bcl–2, widely known for its characteristic to transport the GSH from the cytosol to the nucleus,protecting cells against mitochondrial oxidative stress and subsequent apoptosis.Here, we have validated the antioxidative effect of genistein on rat model (rattusnorvegicus) where oxidative stress was induced by D–GalN treatment. Genistein significantly attenuated the effects of D–GalN and levels of marker enzymes like AST, ALT and BCL–2 which were reestablished in the animals pretreated with it. We also introduce a computationally comprehensiveprotein 3–D model of Bcl–2 of rattusnorvegicus by using CPH model, SAVES and SWISS–MODEL workspace (http://www.cbs.dtu.dk/services/CPHmodels,http:// nihserver.mbi.ucla.edu/SAVES/,http://swissmodel.expasy.org/qmean/cgi/index.cgi)and it was validated withvarious computational tools like ERRAT, PROCHECK, VERIFY 3D etc. Wealso present docking study to check the interaction between the Bcl–2 and genistein with the help of web based serverpatch dock, an online tool for Ligand Protein Docking (http://bioinfo3d.cs.tau.ac.il/PatchDock).Docking score between Bcl–2 and genistein was found to be165.38 kcal/mol, which validates the interaction between them. Therefore, it is proposed that the genistein works on to the Bcl–2 to increase the uptake of GSH from cytoplasm to nucleus.