EVALUATION OF SERUM FERRITIN LEVEL AND IRON REGULATORY PROTEIN-1 GENE EXPRESSION IN PATIENTS WITH CHOLELITHIASIS

Dr. Deepanshu Aggarwal; Dr. Vivek Agrawal; Dr. BD Banerjee

Volume : VIII, Issue : XII, December - 2019

EVALUATION OF SERUM FERRITIN LEVEL AND IRON REGULATORY PROTEIN-1 GENE EXPRESSION IN PATIENTS WITH CHOLELITHIASIS

Dr. Deepanshu Aggarwal, Dr. Vivek Agrawal, Dr. Bd Banerjee

Abstract :

Background: Cholelithiasis is a major problem affecting females, which can be attributed to iron deficiency anaemia. Aim: To evaluate serum ferritin level and iron regulatory protein-1 (IRP-1) gene expression in patients with cholelithiasis and compare mRNA expression of IRP-1 gene in patients of cholelithiasis with their first degree relatives. Methods: 55 patients of cholelithiasis and 55 controls were included, and a fasting blood sample was taken for evaluation of S.ferritin levels and IRP-1 gene expression. The patients were divided into anaemics and non-anaemics. S.ferritin level of these groups were compared and IRP-1 gene expression of patients was compared with their respective controls. Result: 92.7% (51/55) patients were females. 61.8% of the patients (34/55) were anaemic. Out of these 34 anaemic patients, 33 (97.1%) were females. The p-value of ferritin (0.043) was less than 0.05 indicating a significant change in ferritin values in the two groups. 1.22 fold change upregulation was observed when all patients were considered as a test group and all relatives as controls. Conclusion: Significant decrease in S.ferritin was found in anaemics with cholelithiasis. IRP-1 gene showed upregulation in all anaemics with cholelithiasis as compared to controls.