Evaluation of Gene Xpert Mycobacterium tuberculosis Assay for the Detection of Rifampicin Resistant Mycobacterium tuberculosis among Pulmonary Patients: A Hospital Based Study

Dr Basavaraj M S

Volume : VI, Issue : I, January - 2017

Evaluation of Gene Xpert Mycobacterium tuberculosis Assay for the Detection of Rifampicin Resistant Mycobacterium tuberculosis among Pulmonary Patients: A Hospital Based Study

Dr Basavaraj M S

Abstract :

Conventional diagnostic methods for Mycobacterium tuberculosis are slow and/or lack sensitivity. A number of new diagnostic approaches have ought incremental improvements to detection and drug susceptibility testing; however, the technical complexity of these assays and their dependence on dedicated laboratory infrastructure have limited their adoption, especially in low-resource, high-burden settings.It ranks as the second leading cause of death from an infectious disease worldwide. My aim was to evaluate the performance of GeneXpert system for the detection of Mycobacterium tuberculosis with its Rifampicin resistance. The concentration of M. tuberculosis bacteria in clinical sputum samples can vary from over 107 to less than 20 CFU/ml.Sputum samples were collected from 135 clinically suspected multidrug resistant pulmonary tuberculosis (MDR-TB) patients.