EFFICACY OF RAPID IMMUNOCHROMATOGRAPHIC TEST IN THE DIAGNOSIS OF HEPATITIS B.

Ingle R; Kumar Chaya A; Chavan S; Baveja S

Volume : IX, Issue : VI, June - 2020

EFFICACY OF RAPID IMMUNOCHROMATOGRAPHIC TEST IN THE DIAGNOSIS OF HEPATITIS B.

Ingle R, Kumar Chaya A, Chavan S, Baveja S

Abstract :

Introduction: Hepatitis B (HBV) is a hepatotropic viral disease causing complications like fulminant hepatitis, cirrhosis of liver and liver cancer. The standard method for diagnosis of Hepatitis B is detection of HBsAg by ELISA. But in emergency conditions and in primary healthcare centers an efficient rapid test is required for Hepatitis B diagnosis. Aims & objectives: To assess efficiency of rapid test to ELISA in detection of HBsAg in the diagnosis of Hepatitis B in general population at a tertiary health care center. Method: A prospective, cross sectional study was conducted for a period of one year at a tertiary care hospital and included 600 subjects of all age groups. Five milliliter of blood was collected for testing Hepatitis B by both rapid (HBsAg Trustline, Chennai, India) & ELISA (HBsAg MERILISA, Gujarat, India) tests. Results were analyzed statistically. Result: HBsAg Rapid test gave a sensitivity of 94% & specificity of 100% with a PPV of 100% & NPV of 99.83%, taking ELISA as the gold standard. Conclusion: The Rapid test(HBsAg Trustline) was found to be an efficient point of care test in the diagnosis of Hepatitis B and could be used in healthcare setup where rapid and accurate results are desired.