Effect of malathion, dimethoate and chlorpyrifos on Cabbage Peroxidase (Brassica oleracea) activity.

Ila Bania; Dr. Rita Mahanta

Volume : IV, Issue : VIII, August - 2015

Effect of malathion, dimethoate and chlorpyrifos on Cabbage Peroxidase (Brassica oleracea) activity.

Ila Bania, Dr. Rita Mahanta

Abstract :

This study focused on the effect of Malathion, Dimethoate and Chlorpyrifos on Cabbage peroxidase activity. H2O2- mediated oxidation of O-dianisidine by Cabbage Peroxidase was used to assess the enzymatic activity. The specific activity of Cabbage peroxidase was determined in presence of five different concentrations (0.2%, 0.4%, 0.6%, 0.8% and 1.0%) of the three tested pesticides. Malathion and Dimethoate were found to inhibit cabbage peroxidase activity whereas, chlorpyrifos showed no significant effect. The values of Km and Vmax in presence and absence of inhibitors were determined from Lineweaver-Burk plot by following the optimum pH and temperature conditions. Kinetic studies showed that the inhibition types were competitive for Cabbage peroxidase in presence of malathion and noncompetitive in presence of dimethoate.