Comparative Optimization Studies of Protease Production by B. cereus NC77, B. subtilis MD2 and B. amyloliquefaciens MD81

Kusuma Dorcas; Pavan Kumar Pindi

Volume : VI, Issue : XI, November - 2017

Comparative Optimization Studies of Protease Production by B. cereus NC77, B. subtilis MD2 and B. amyloliquefaciens MD81

Kusuma Dorcas, Pavan Kumar Pindi

Abstract :

Bacteria play a vital role in technology for the production of extracellular enzymes especially proteases which are widely used on an industrial scale in many laundry industries. Soil isolates were screened in casein agar and later shake flask method was used for protease production. Out of 98 isolates, eight were found to be good protease producers. Of which 3 isolates showed maximum protease production viz., B. cereus NC77, B. subtilis MD2, B. amyloliquefaciens MD81 which initially produced proteases of 211U/ml, 175U/ml and 128U/ml respectively. There is increasing demand for enzymes and the need for such economically useful enzymes. An optimization study was carried out using various parameters like incubation period, inoculum size, p^H, temperature, carbon sources, nitrogen sources, metal ions using these three isolates. There was a 4.3 fold rise in protease production with Bacillus cereus NC77, an 8 fold rise in production was observed with Bacillus subtilis MD2 and a 9 fold rise in production with Bacillus amyloliquefaciens MD81 was observed. This protease was used in the removal of blood stains effectively in very less time, low concentrations and hence maybe considered the major concern in many hospital laundries for destaining the blood stained clothes as effective detergents.