Comparative evaluation of Hybrid capture 2 and GeneXpert for detection of High risk Human papillomavirus in patients with oropharyngeal squamous cell carcinoma at SMS Hospital, Jaipur.

Dr. Rahul Agarwal; Dr. Jitendra Tiwari; Dr. Bharti Malhotra

Volume : VIII, Issue : VII, July - 2019

Comparative evaluation of Hybrid capture 2 and GeneXpert for detection of High risk Human papillomavirus in patients with oropharyngeal squamous cell carcinoma at SMS Hospital, Jaipur.

Dr. Rahul Agarwal, Dr. Jitendra Tiwari, Dr. Bharti Malhotra

Abstract :

Background: Head and Neck Squamous Cell Carcinoma (HNSCC) is the sixth most common cancer and the eighth most common cause of death in cancer worldwide. Its important to carry out regular HPV testing on such patients as HNSCC which are HPV (Human papillomavirus) positive respond better to radiotherapy. Methods: A total of 50 oral ushing samples were collected in specimen transport medium (STM) from patients with oropharyngeal squamous cell carcinoma. The HR-HPV (High risk Human papillomavirus) was detected by Hyid Caputre 2 (HC 2) HPV system (Qiagen) which is a nucleic acid hyidization based method and GeneXpert system (Cepheid) which is a point of care real-time Polymerase Chain Reaction (PCR) assay. Results: Out of 50 oral ushing samples, 08/50 (16.00%) samples were found positive for HR-HPV by HC 2 and 05/50 (10%) by GeneXpert. Among 05 samples positive by GeneXpert, 4 were HPV 16 positive and 1 sample was other than type 16,18 and 45. However HC2 could not differentiate between the types. Conclusion: Qiagen Hyid capture 2 is a well established method but is cumbersome and time consuming, newer methods like GeneXpert are easy to perform and give results in 1 hour but needs to be compared with established methods.