Chromogenic Spray for Detection and Identification of Paraphenylenediamine from Biological Material

Charansing B. Ghoti; Keshav S. Kapgate; Shivangi S. Apte; Vijay J. Thakre

Volume : IX, Issue : II, February - 2020

Chromogenic Spray for Detection and Identification of Paraphenylenediamine from Biological Material

Charansing B. Ghoti, Keshav S. Kapgate, Shivangi S. Apte, Vijay J. Thakre

Abstract :

Thin layer chromatography (TLC) is a simple, rapid and reliable technique usually used in forensic science laboratory for detection of poison in biological material. It can separate many complex mixtures in a short period of time. In this study an effort has been taken to determine Paraphenylenediamine by using Thin Layer chromatography. A specific chromogenic reagent Ninhydrin in ethanol has been used for the detection of Paraphenylenediamine with Chloroform: Acetone (1:1) as a solvent system for the separation