Bacteriological profile of neonatal sepsis and pattern of antimicrobial susceptibility in a tertiary care hospital of Amritsar

Kiranjeet Kaur; Kanwardeep Singh; Karnail Singh; Pushpa Devi

Volume : IV, Issue : VII, July - 2015

Bacteriological profile of neonatal sepsis and pattern of antimicrobial susceptibility in a tertiary care hospital of Amritsar

Kiranjeet Kaur, Kanwardeep Singh, Karnail Singh, Pushpa Devi

Abstract :

Objective: To study the bacteriological profile of neonatal sepsis and pattern of antimicrobial susceptibility in a tertiary care hospital of Amritsar from January 2013 to June 2014. Methods: The study was done on all the neonates admitted with clinical signs of septicemia. Blood samples were collected using aseptic precautions and subjected to bacteriological culture and antimicrobial susceptibility testing using standard established techniques. Results: Of the total 512 clinically suspected cases of sepsis, bacterial agents were isolated from 232 (45.3%) cases. Percentage of Gram positive organisms was 33.2% and of Gram negative organisms was 66.8%. Most common isolate was Klebsiella pneumonia followed by Coagulase Negative Staphylococci (CONS). The most common isolate in Early Onset Sepsis (EOS) was CONS and in Late Onset Sepsis (LOS) Klebsiella pneumonia. Gram positive organisms were most sensitive to linezolid and amikacin whereas Gram negative organisms showed maximum sensitivity to imipenem and combination of ceftizidime-sulbactam. Risk factors associated with neonatal sepsis were low birth weight (64.2%), preterm birth (43.7%), premature rupture of memanes (PROM) (23%), home delivery (19.2%) and maternal fever (17.3%). Conclusion: Multidrug resistant Gram negative isolates are frequently encountered in cases of neonatal sepsis. Good patient care practices and rational use of antibiotics can go a long way to reduce infection rate in neonates and to ensure better therapeutic success.